The Receptor for Advanced Glycation End Products (RAGE, UniProt ID Q15109) is a type I integral membrane protein that binds advanced glycosylation end products (AGE). The 50–55 kDa glycosylated protein contains an extracellular domain (aa 23–342), a hydrophobic transmembrane domain (aa 343–363), and a cytoplasmic domain (aa 363–404). The RAGE extracellular region is composed of a variable (V) immunoglobulin domain (aa 23–116) and two constant Ig domains (C1, aa 124–221; C2, aa 227–317) connected by a flexible seven amino acid linker. RAGE is associated with several diseases, including diabetic complications, Alzheimer's disease, cancer, Lupus, and hypertension. Antibody B0077-C03-D11 binds specifically to the C2 domain of the human RAGE protein, demonstrated using a dot-blot screen and an ELISA against purified V, C1 and C2 domains of RAGE. The antibody binds to purified RAGE extracellular domain with KD = 0.3 nM, measured using high throughput SPR (Figure 5).